Author(s): M. Rajikkannu, Nirmala Natarajan, P. Santhanam, B. Deivasigamani, J. Ilamathi, S. Janani

Abstract: The present study was carried out to evaluate the influence of dietary supplementation of probiotic bacteria (Bacillus pumilus) on haematological parameters of Labeo rohita. The Probiotic was isolated from the intestine of Indian major carp (Labeo rohita). The feeding trail was conducted for 60 days. The fish with similar body weight (5±1gm) were distributed randomly into three treatment groups, which fed a feed containing Bacillus licheniformis, Bacillus cereus and Bacillus pumilus in T1, T2, and T3. The control group (T4) was fed without probiotics for the same period. Blood samples were collected at the intervals 0, 15, 30, 45 and 60 days. The haematological parameters such as Total erythrocytes count (RBC), Haematocrit (Hct), Haemoglobin concentration (Hb), and Haematological indexes (MCV, MCH and MCHC) were examined. The Bacillus pumilus treated fish (T3) showed maximum percentage Totalrnerythrocyte count, haemoglobin concentration and haematocrit concentrations than in other groups. The result suggests that Bacillus pumilus could be used effectively as a probiotics for the use in aquaculture.

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