Author(s): Anjni Kumari, Ajit Kumar, Shakti Kumar

Abstract: An experiment was conducted for 60 days to assess the probiotic potential of Bacillus licheniformis, a Gram-positive, aerobic, spore forming bacterium in climbing perch, Anabas testudineus juvenile having average weight of 10±2 g. fifteen rectangular fiber tanks of 100 liters capacity were taken for study and each tank was stocked randomly with 12 fishes. Treatment groups T1, T2, T3 and T4 were fed with four different diet containing (1.0 x 107 cfu g-1, 0.5 x 108 cfu g-1, 1.0 x 108 cfu g-1, and 0.5 x 109 cfu g-1 diet respectively) B. licheniformis for 60 days and the control group (C) was fed with diet without B. licheniformis for the same period. The growth, haematological and serum parameters were monitored at fortnight interval. In treatment groups the weight gain (WG) and specific growth rate (SGR) were significantly high (p<0.05) in comparision to control. Fishes fed with diet containing 1.0 x 108 cfu g-1 B. licheniformis showed highest weight gain (87.47±2.98%) and SGR (1.47±0.03). The TEC and haemoglobin content increased significantly in treatment groups in comparision to control group. The highest TEC (2.08±0.04 x 106/mm3) and haemoglobin content (7.47±0.02 g%) was observed in T4. The present study suggests that B. licheniformis at 1.0 x 108 cfu g-1 feed could be used in climbing perch farming to enhance growth and haematological parameters.

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