Author(s): Muhammad Forhad Ali, Md. Mamunur Rahman, Md. Khairul Bashar, Rabeya Rahmatullah, Md. Hadiuzzaman and Md. Ruhul Amin

Abstract: Induced breeding of shing, Heteropneustes fossilis (Bloch, 1794) was carried out by using pituitary gland (PG) and human chorionic gonadotropin (HCG). Twenty pairs of brood fishes were reared up to maturation for spawning operation in a fish farm by providing farm-made artificial diet containing 30% protein for 4-5 months before onset of breeding season. The breeders were induced with hormone HCG (Male 1250 IU/kg and female 2000 IU/kg), PG (Male 10 mg/kg and female 70 mg/kg), HCG 1250 IU/kg for male and PG 70 mg/kg for female, PG 10 mg/kg for male and HCG 2000 IU/kg for female for treatments T1, T2, T3 and T4, respectively. The brood fishes were injected with a single dose. When the brood fishes were injected with HCG the breeding behavior was exhibited quickly and perhaps males lost most of their milt before the ovulation of female and resulting less fertilization rate (84%) in T1­. Whereas, the male and female fishes were injected with PG the eggs and milt released at the contemporary times, resulting highest fertilization rate (95%) in T2. In other combination there was some variation of synchronization of releasing eggs and milt. Fertilization rate was 84%, 95%, 80% and 89% and hatching rate was 81.40%, 93%, 89% and 68.90% in treatments T1, T2, T3 and T4, respectively. The incubation periods for fertilized eggs were 22-24 hrs in all treatments. Higher fertilization rate (95%) and hatching rate (93%) were found in case of PG-PG combinations in T2. For induced breeding of H. fossilis a dose of PG 10 mg/kg body weight of male and 70 mg/kg body weight of female in T2 was found to be most effective among the combinations of hormones used.

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