International Journal of Fisheries and Aquatic Studies
2013, Vol. 1, Issue 1, Part A
Tissue and Substrate Specific Esterase Isozyme Banding Pattern in Macrobrachium rosenbergiiAuthor(s):
Md. Abdur Rashid, Mohammad KamruzzamanAbstract:
Tissue and substrate specificity of esterase isozymes was studied from five different tissues of Macrobrachium rosenbergii on polyacrylamide gels (7.5 %) stained with α or β or both α and β naphthyl acetates together. Altogether, five esterase bands (Est-1, Est-2, Est-3, Est-4 and Est-5) were observed that showed both tissue and substrate specific expression. Maximum, four esterase bands were found in eye (Est- 1, Est-3, Est-4 and Est-5) and tail muscle (Est-1, Est-2, Est-4 and Est-5) while other tissues showed three bands stained with both α and β naphthyl acetates together. Est-5 was highly expressed (100%) in all the tissues as well as in all three substrates (α or β or α-β naphthyl acetates together). Most of the bands were found to be α-β specific (68 %) while few bands were α (28 %) or β (36%) specific. Both Est-1 and Est-4 were completely absent in α as well as in β stained gels.Pages: 14-17 | 772 Views 14 DownloadsDownload Full Article:
How to cite this article:
Md. Abdur Rashid, Mohammad Kamruzzaman. Tissue and Substrate Specific Esterase Isozyme Banding Pattern in Macrobrachium rosenbergii. International Journal of Fisheries and Aquatic Studies. 2013; 1(1): 14-17.